There are a few things to keep in mind when prepping for an audition that can make your performance all the clearer– one is to be aware of your eye line (meaning where you are looking) in your audition. It can be difficult for actors to remember the technical side of their reading along with the emotional side. The inner work is most important but there are times, say in action scenes where one has to see something or one may have to refer to two characters, maybe notice someone enter a room, etc., where one has to be specific and technical. These things should not be ignored. Pay close attention to the fine details in the stage directions.
For your opening lines, be sure to say them outward and not looking down at the page. It helps leave a strong, memorable opening impression.
Also, it's important to not go over the top with physical directions in the audition, like if one has to pretend to use a weapon and whack someone for a film audition, be calculating with it and don't thrash around outside of the frame. For a theatre audition, you may move around a bit but not too much, don't overdo it– staging comes in rehearsal and don't give the person auditioning you your back of course, maybe for a second or two (if ever at all), or only if it's in the stage directions. It's important to never invade a casting director or director's space when doing your audition, it won't impress them and they'll think you might be crazy.
After the audition is over, stay positive and grateful you've had the opportunity to audition. Take every audition as a chance to leave an impression to be considered in the future. Nothing is ever wasted.
Break a leg!